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the space between the oxygen atom of Thr164 of Aph1 as well as oxygen atom of Tyr466 of PS1 is predicted to get three.five Å, suggesting that more info a hydrogen bond (H...O length, roughly 1.eight Å) is formed in these aspect chains (determine 7b). The T164A mutation may well reduce the hydrogen bond with PS1 Y466, causing adaptable motion in the carboxy terminus of PS1. We propose that Aph1 mutations regulate protease action by conformational alterations in PS1.

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